Label-free analysis workflow. The first step in the workflow of label-free cell cycle classification is to acquire brightfield and darkfield images from the cells (see Methods section).To allow ...

DAPI (1ug/ml), Hoechst 33342 (10 ug/ml), PI (50 ug/ml) or 7-AAD (25 ug/ml) added and cells analysed for cell cycle analysis. DAPI and Hoechst 33342 can also be used to determine the cell cycle of ...