Numerous DNA binding viability dyes can be used in the annexin V assay including PI, DAPI and DRAQ7 (Biostatus). The Millipore Muse mini flow cytometer can be used to determine apoptosis levels in a ...
It can detect early stage apoptotic change, generally in combination with a viability dye (e.g., 7-AAD or PI). When conjugated with fluorescein isothiocyanate (FITC), this dye labels ...
A pathologist determines the cell viability by manually ... Fluorescent dyes, such as acridine orange (AO) and 4',6-diamidino-2-phenylindole (DAPI), overcome this limitation by selectively ...