The Nuclear-ID Red dye was imaged using a Texas Red filter set, and the green fluorescence was imaged using a FITC filter set, on an Axio Observer Z1 wide-field fluorescence microscope equipped ...

For fluorescence microscopy, the core offers the Biotek Cytation 5 equipped with standard DAPI, FITC, RFP, Texas Red, CY5 and CY7 filters and a range of magnifications from 2.5x to 60x air lenses. The ...

But FITC has considerable spillover into the PE ... In testing a multicolor reagent cocktail, it is wise to include fidelity and fluorescence-minus-one controls in the initial testing 2.