in order to cut the insert (=the gene) from the plasmid DNA (=vector). Following separation of insert and vector by electrophoresis, you will elute the insert from the agarose. More background ...

Figure 1: Amplification of complex gene libraries by conventional PCR and emulsion PCR. Figure 2: Ethidium bromide–stained agarose gels reveal the absence of size competition and chimeric produc ...

pMRBAD-link-CGFP uses the kanamycin resistance gene as a selectable ... Purify the vector products by electrophoresis through 0.8–1% agarose and determine the concentration of vector DNA by ...