These short probes are highly specific and possess the high melting temperature (Tm) required for real-time PCR owing to the incorporation of locked nucleic acid (LNA). The probes are dual-labeled ...

Locked nucleic acid (LNA) probes Probes that use modified nucleotides with a locked ribose ring, increasing the thermal stability of the probe-target hybrid While their specificity and thermal ...

However, under the low stringency conditions required for short nucleic acid probes to form stable interaction, the longer single-stranded targets are able to engage in secondary structure.

More information: Sungho Kim et al, Fast and accurate multi-bacterial identification using cleavable and FRET-based peptide nucleic acid probes, Biosensors and Bioelectronics (2024). DOI ...

1 Roughly a decade later, research by Higuchi et al. led to the discovery that PCR amplification can be monitored in real time using fluorogenic nucleic acid probes, allowing for quantitative ...

Researchers from Agency for Science, Technology and Research in Singapore recently published a review article exploring how chromatography can be used to remove double-stranded RNA impurities during ...

Steven Suljak and his research assistants are building better tools for studying chemical and biological systems. Microcolumn separations of proteins, nucleic acids, and other biomolecules.