Overlay PCR reaction with 15 μl of mineral oil to prevent evaporation during the temperature cycling. Other DNA polymerases can also be used for this preamplification, depending on the fidelity ...

thus enhancing PCR amplification for templates with high GC content. Usually, scientists use a concentration range of 1.25-10 percent. BSA can alleviate the inhibitor effect of ferric salts and ...

Polymerase chain reaction (PCR)-based rapid sterility testing offers a revolutionary alternative, delivering accurate contamination detection in hours while maintaining the sensitivity needed to ...

Pool the emulsified PCR reactions in a 1.7-ml microcentrifuge ... Perform the following extraction twice: add 1 ml of water-saturated diethyl ether, vortex the tube, and dispose of the upper ...