They are essentially used to normalize the levels of protein detected within a sample by ensuring that protein loading is the same across the gel. Below, the four main reasons why loading controls ...

3. The gel. A protein gel is formed in two sections, the stacking gel and the resolving gel. The role of the stacking gel is to allow sample loading and to guide the samples into the top of the ...

For native gel electrophoresis, individual fractions of our protein complex enrichment procedure were mixed 1:5 with loading buffer and loaded on a 3–8% Tris-Acetate native gradient gel with 1 ...

Among the endoproteases used for protein digestion, serine protease trypsin is the most common one, as it generates peptides that are highly amenable to MS analysis. Depending on the preceding ...

The finding could change the way protein therapeutics are formulated, possibly eliminating the need for cold storage (Nature 2024, DOI: 10.1038/s41586-024-07580-0).

Running gels and protein powders can be convenient boosts for athletes – but be sure to read the label Published: April 12, 2023 10:02pm EDT. Emma ...

Researchers have developed a protein-based gel that breaks down alcohol in the gastrointestinal tract without harming the body. In the future, people who take the gel could reduce the harmful and ...

The use of loading controls helps to normalize the levels of protein that are detected in the sample, as they provide proof that the protein has been uniformly loaded across the gel. The levels at ...