Regardless of source, the target protein must be suspended in a buffer similar to TGMC(0.1) for micrococcal nuclease treatment. Add 1 U of micrococcal nuclease to each tube of 30 μl of ...

and there also are a variety of perdeuterated buffers (d-Tris, d-HEPES) available that do not obscure protein NMR signals. Protein preparation for NMR gives further details on how to optimise the ...

Commercial products which buffer more quickly than alfalfa are ... The higher calcium and protein content in alfalfa is what ...

Rock the immunoprecipitate at 4 °C for another 30 min. 5| Wash the protein A–Sepharose mixture in NETN buffer (20 mM Tris (pH 8), 1 mM EDTA, 900 mM NaCl, 0.5% NP-40). Repeat this wash five more ...