Thus, the overall efficiency of RT– and real-time PCR in detecting the housekeeping gene in a limited number of picked cells was 82% (243/297 samples). Negative samples may be mostly caused by ...

ProbeLibrary, a fast, specific and flexible format for quantitative real-time PCR, is described. The ProbeLibrary concept is based on the fact that just 90 short probes provide transcriptome-wide ...