Real time RT–PCR is a nuclear-derived method for detecting the ... The mixture is then placed in an RT–PCR machine. The machine cycles through temperatures that heat and cool the mixture to trigger ...

or real-time RT–PCR. Fast and accurate, real-time RT–PCR has been critical in detecting, tracking and studying COVID-19. But what is it, and how does it work? Let us explain.

Other popular machines for real-time PCR include Bio-Rad's iCycler IQ, a 96-well machine that can detect four dyes, and Stratagene's Mx3000P, a lower-cost personal thermocycler. Many companies ...

PCR-Machines are based on Polymerase Chain Reaction (PCR) and produce copies of genes exponentially. The PCR cycling reactions are carried out in an automated thermocycler, which is capable of ...

We have developed a two-step, real-time, quantitative assay, using the polymerase chain reaction with reverse transcription (RT-PCR) and based on SYBR Green I monitoring of PCR product ...