Since the emergence of COVID-19 in late 2019, the gold standard in testing for the disease has been a nuclear-derived technique: real-time reverse transcription–polymerase chain reaction, or real-time ...

This extension of real-time PCR technology, called reverse transcription PCR (RT-PCR), combines real-time PCR with reverse transcription, the process that makes DNA from mRNA. RT-PCR can be used ...

Current molecular assays that are based on nested reverse transcription PCR and real-time PCR provide tools for follow-up of hepatitis A infection. The nucleic acid sequencing of selected regions ...

is a commonly used molecular biology lab technique to determine the actual amount of PCR product at a given cycle. For quantitative reverse transcription PCR (RT-qPCR), the starting material is RNA, ...

sensitivity and inhibitor resistance in reverse transcription loop-mediated isothermal amplification (RT-LAMP), RT-PCR, and other applications where the detection of low-quantities of target RNA from ...

In order for a virus like the COVID-19 virus to be detected early in the body using real time RT–PCR, scientists need to convert the RNA to DNA. This is a process called ‘reverse transcription’. They ...

real-time PCR (qPCR), digital PCR (dPCR), reverse transcription PCR (RT-PCR), hot-start PCR, multiplex PCR, and other PCR techniques. The qPCR segment holds the largest share of the market.

Until this past January, few outside academic and diagnostic labs knew what quantitative reverse-transcription PCR (RT-qPCR) was, much less its role in viral and bacterial disease detection.

Then, the DNA is examined by the PCR method using virus-specific primers to determine whether the sample has that specific virus. This is quantitative reverse transcription PCR (RT-qPCR), which means ...

The two tests – reverse transcription polymerase chain reaction (RT-PCR) and antigen rapid test kit (RTK-Ag) – require nasal or throat swab samples. Cancel anytime. No ads. Auto-renewal.