qPCR, also known as real-time PCR ... 6 Ideally, primers should be 15-30 base pairs long (optimal for short amplicons, 70-200 bp). Design forward and reverse primers with similar melting temperatures ...

The addition of a reverse transcription (RT) step to qPCR occurs when the target is ... researchers needed to find the best locations for PCR primers and probes on viral RNA.

Several components are involved in a PCR including template DNA, forward and reverse primers, DNA polymerase enzyme, and reaction buffer. Scientists must ensure that all reagents in the reaction are ...

The GoTaq ® Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and ...

With proprietary LNA™ technology, the miRCURY™ LNA™ miRNA PCR System takes your qPCR experiments to the next level ... and specificity afforded by LNA™-enhanced primers with a universal reverse ...

RT-qPCR, reverse transcription qPCR; SCFA, short-chain fatty acid; SV, sham vehicle. Credit must be given to the creator. Only noncommercial uses of the work are permitted.