Antibiotic resistance genes were typically isolated by cloning from cultured bacteria or by PCR amplification. Those methods missed potential antibiotic resistance reservoirs because of ...

Molecular cloning refers to the process of creating multiple copies of a specific gene or DNA sequence. This is typically done using bacteria or other microorganisms as host cells. The legal status of ...

In principle, cloning PCR products necessitates some fancy ... We are aiming to understand what, if any, bacterial proteins are involved in Red recombination.” In the lab of Kenan Murphy ...

Select and verify by sequencing at least six Cm R /Km R bacterial clones for each individual target oligonucleotide when cloning individual shRNAs, or ensure at least threefold coverage when ...