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Nature5d
Manufacture of T cells using the Sleeping Beauty system to enforce expression of a CD19-specific chimeric antigen receptor
This assay is accomplished over 18 days to reveal ... which combines CliniMACS Separation Technology with cell processing capabilities to automate cytokine capture, 54 has been successfully used ...
Nature6d
Continuous requirement for the TCR in regulatory T cell function
For cytokine staining, lymph node and spleen cells were stimulated for 4–6 h at 37 °C, 5% ... In vitro IL-2 capture assay. Trac FL/WT Foxp3 Cre-ERT2 mice were administered tamoxifen on days 0 ...
gut.bmj6d
A large population study of spontaneous HBeAg seroconversion and acute exacerbation of chronic hepatitis B infection: implications for antiviral therapy
After HBeAg seroconversion, 65.2% (73/110) of patients had undetectable HBV DNA levels by the Digene Hybrid Capture assay. Of these, 78.1% still had HBV DNA levels detectable by the Amplicor HBV ...
Frontiers3d
Simplified Flow Cytometry-Based Assay for Rapid Multi-Cytokine Profiling and Machine-Learning-Assisted Diagnosis of Inflammatory Diseases
Multiple cytokine detection represents a more robust way to predict the disease progression than a single cytokine, and flow cytometry (FCM)-based assays are increasingly used worldwide for multiple ...