GFP-LC3, mito-RoGFP, mtKeima, LTR, and MTG/TMRE flow cytometry were done as described (25), and FlowJo 10.3 software was used for gating analysis and quantification for all flow cytometry studies.

Briefly, high resolution confocal images containing the LC3 (red and green) and CTSD (blue) punctate signals were analyzed with the Zen Blue Image Analysis Module from Carl Zeiss Microscopy. Threshold ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Small GTPase RHEB is a well-known mTORC1 ...

FAM134B2 lacks the first hairpin structure of the reticulon homology domain but retains the LC3-interacting region (LIR) domain, enabling it to function as an ER-phagy receptor. Proper ER membrane ...

Masroor Ahmad Paddar and colleagues reveal noncanonical roles of ATG5 and membrane ATG8ylation in regulating retromer assembly and function. They identify ATG5's unique non-autophagic role and show ...

LC3 (Little Computer 3) assembly programs that I created for "Introduction to Computing" with Dr. Yale N. Patt Not all of these programs are perfect solutions! LAB-4: NIM is the main project in this ...

1Cellular Degradation Biology Center, College of Medicine, Seoul National University, Seoul, Republic of Korea. 2Department of Biomedical Sciences, College of Medicine, Seoul National University, ...

To do so, we performed long-term live imaging of GFP-Rab10 using digital holotomographic microscopy (DHTM) in combination with epifluorescence, confocal imaging of known Rab10 interactors and ...