The Anatrace HiFliQ-NiNTA Column is pre-packed and ready to use with pre-charged Nickel-NTA agarose resin for ... proteins by immobilized metal ion affinity chromatography (IMAC).
The nanobodies were expressed and purified from mammalian cells using Ni-NTA affinity chromatography and gel filtration. Their purity and concentration were confirmed through spectrophotometry and ...
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