Quantitative PCR with no gel? Impossible! Now with the ABI PRISM 7700 Sequence Detection System, real time PCR has become a reality. Combining a novel chemistry with a fully integrated PCR ...

Endpoint analysis: Scientists analyze PCR products typically using agarose gel electrophoresis. Real-time PCR detects and quantifies DNA as the reaction progresses. This quantifies the specific amount ...

Clean the emulsified (Step 9) and nonemulsified (Step 6) PCR products using the Wizard SV Gel and PCR Clean-Up System. Problem: A coarse emulsion is formed. Low-quality emulsions can be identified ...

At the completion of the reaction, amplification products are analyzed by size fractionation using gel electrophoresis. Real-time PCR follows the same course, except that the products are detected ...