The use of electrical impulses also speeds up the purification process, Ferrand-Drake del Castillo says, explaining that “instant millisecond switches reduce the cycle time and use of raw materials by ...
Cell pellets were resuspended in four times the pellet volumes of lysis buffer (50 ... Figure S2. Purification and structure determination of HsIFT172 C-terminal domain. (A) SEC elution profile for ...
NS0-derived mouse CLEC4F/CLECSF13 protein ... and the buffer was exchanged from 0.5 M imidazole elution buffer to PBS by dialysis. The purity and size of the nanobody was detected by SDS-PAGE and ...
Expression and purification of ... supernatants in a binding buffer (100 mM Tris–HCl pH 8.4, 0.15 M NaCl, 1 mM EDTA) were applied to a StrepTrap column (QIAGEN, Hilden, Germany). After × 10 washing, ...
In this installment, I primarily discuss considerations to keep in mind when choosing buffering additives that will be used ...
Asked about a possible deployment of German troops to help secure a buffer zone between Russia and Ukraine if one were agreed, Pistorius said: “We’re the largest NATO partner in Europe. We’ll ...
The journey to develop an upstream bioprocess is complex, involving process development, optimization and scale-up. Hence, it’s important to choose the right equipment to accelerate this journey while ...
Transcriptional regulation governs gene expression levels, primarily controlled by “cis-acting DNA elements” and “trans-acting protein factors”. However, the conventional view that cis-regulation is ...
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