The molecular hallmark of SFTs is a gene fusion between the NAB2 and STAT6 loci on chromosome 12, resulting in a chimeric protein of ... times with co-IP buffer and one time with PBS with 0.05% NP-40.

The target proteins were detected by SDS-PAGE. For carrying out the denaturing purification of the proteins, 8 M urea was added to the lysis buffer, wash buffer, and elution buffer. The eluted protein ...

Transcriptional regulation governs gene expression levels, primarily controlled by “cis-acting DNA elements” and “trans-acting protein factors”. However, the conventional view that cis-regulation is ...

Challenging is the loss of nucleic acids during necessary purification processes, since elution off solid surfaces is not efficient ... and adenovirus in buffer followed by qPCR detection. This ...

Extracellular vesicles (EVs) are now recognized as powerful modulators of metabolism, and thus the new field of EV-mediated metabolic regulation is growing exponentially. Here, we discuss special ...

The protein bands were cut out, placed in Eppendorf tubes, and destained with Bio-Rad destaining buffer. In-gel trypsin digestion was performed as described 18. Oxidation of proteins. Quantitative ...

Methods to affinity purify protein complexes and to determine ... and 740 nm light for elution. Far-red light-induced elution is effective but very mild as the same buffer is used for the wash and ...