Improving the detection of small RNAs in fecal samples paves the way for novel, non-invasive biomarkers to monitor intestinal ...

Then, we further used the TaqMan MicroRNA Transcription Kit (Applied Biosystems, Foster City, CA, USA) to prepare the RNA samples. The sequence and Assay ID for each miRNA kit are available in ...

Until recently, the use of target prediction algorithms followed by validation of direct miRNA targeting of selected 3′-UTRs in a cell culture transfection assay was the primary way to establish miRNA ...

†: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, ...

miR microarray was performed using the Human MiRNA Genome V2.0 Complete RT 2 PCR Array ... firefly luciferase was measured in cell lysates using the britelite plus Reporter Gene Assay System ...

Dublin, May 21, 2025 (GLOBE NEWSWIRE) -- The "miRNA Sequencing and Assays - Global Strategic Business Report" report has been added to ResearchAndMarkets.com's offering. The global market for ...

The quantification of miR-124 expression was performed using TaqMan MicroRNA Assays (miR-124 Assay ID: 001182; U6 snRNA Assay ID: 4427975; Thermo Fisher Scientific, Waltham, MA, USA). RNA samples were ...

From the resulting differential signatures, the researchers developed a miRNA assay and tested it using RT-qPCR. They verified the resulting miRNA assay in patients with OSSN and PTG as determined by ...