Using an unbiased approach, this important study discovered a role of Ezh2 in the differentiation of granule neuron precursors, the cell of origin for Shh group of medulloblastoma. Furthermore, the ...

PASs were localized using the polyA_DB for the hg38 version. The relative expression difference (RED) was calculated as the difference in log2(RPM ratio) between two groups. To clarify the identity of ...

The mRNA containing PolyA (polyadenylate) was specifically captured through two rounds of purification. The captured mRNA was then fragmented. The fragmented RNA was synthesized into cDNA by reverse ...

RNA sequencing (RNA-seq) libraries were prepared from 500 ng of total RNA using the Illumina TruSeq Stranded mRNA Library preparation kit which allows strand-specific sequencing. A first step of polyA ...

Following the COVID-19 pandemic, mRNA-based vaccines have been shown to provide rapid and precise immune responses, however, this technology has much wider applications. Many leading pharma and ...

CureVac and partner GSK are, meanwhile, also developing an mRNA-based flu vaccine, but are a little further back in development, as their shot is in phase 1 testing.

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