The electrode can now be placed in the pH 7.00 calibration buffer. After waiting for the stable reading for 1-2 minutes, the temperature corrected reading can be recorded.

To determine the optimal concentration of the buffer system, dilute the nanosensor to 16 mg/ml and mix it at a 1:1 (vol/vol) ratio with buffers A (pH 4.5) and B (pH 6.5) to yield a final ...