Levels of proliferation and immunoglobulin class switching to IgG and IgE produced by human B cells were determined, before and up to 10 days after stimulation, by using flow cytometry with mAbs as ...

Methods Differentially labelled streptavidin and extravidin tetramers were conjugated to biotinylated CCP2 or control antigens and used in flow cytometry to ... antigen-specific B cells displayed ...

Mouse B cell line CH12F3, which is a widely used model for the study of B cell activation and class switching (56), was maintained ... The detection of SRBC-specific antibodies was performed by flow ...

Structure of (A) IgG; (B) pentameric ... colleagues performed a class-switch from IgG to IgM with the potent broadly neutralizing HIV-1 antibodies 4E10 [25]. This IgM was recombinantly expressed in ...

For almost six decades, flow cytometry (FC) has been a cornerstone of clinical and laboratory research, with applications in a wide range of fields, including immune-phenotyping, diagnostics, cell ...

To support physicians in treating cancer patients, oncology laboratories can benefit from adopting end-to-end automation of flow cytometry workflows ... multiple myeloma, B-cell acute ...

B-cell prolymphocytic leukemia (B-PLL) is a rare, aggressive type of leukemia that progresses rapidly. People with B-PLL typically have a less favorable outlook. However, new targeted treatments ...

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Researchers have developed a new gene switch that ... blood flow. It is broken down within a few seconds and therefore only affects a very localised area. The implanted cells are modified so ...

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