Bioluminescence imaging involves transducing target cells to express luciferase, a class of enzymes that can efficiently catalyze a reaction that transfers chemical energy into light. The DNA ...

which comes from a luciferase reaction with the luciferin small molecule. And that creates light. And we used those components and dragged them to RNAs of interest. And so when the RNA was produced, ...

The fireflies’ distinctive light is produced through a biochemical reaction involving a molecule called luciferin and an enzyme called luciferase. When these interact in the presence of oxygen ...

Researchers have developed a groundbreaking method to expand the palette of bioluminescent protein colors for cell labeling. By creating 20 distinct colors, they have enabled the simultaneous ...

A team has now identified an enzyme, alpha-kinase 2 (ALPK2), that ... Dec. 6, 2024 — A newly developed luciferase-based reporter can detect problems in protein translocation and disulfide ...

And the insulin concentration in the supernatant was determined by using an insulin enzyme-linked immunosorbent assay (ELISA ... R&D Systems Inc.) according to the manufacturer’s protocol. Dual ...

Dual luciferase reporter assays and chromatin immunoprecipitation ... Results We provide compelling evidence that the glycolytic enzyme PGK1 is repressed by transforming growth factor-β/SMAD4. Loss of ...

The luciferase assay was performed after incubation for 48 h ... Second, due to the amplification nature of the Nluc enzyme, the rSVA-Nluc has a larger dynamic range and higher sensitivity than the ...

Engineered enzymes are poised to have transformative impacts across applications in energy, materials, biotechnology, and medicine. Recently, machine learning has emerged as a useful tool for ...

It is a unique member of the plastic family as it can be broken down into its constituent units (or monomers) by PETases, which are enzymes that degrade PET. Until now, scientists have discovered ...