Flow cytometry enables researchers to label proteins of interest using fluorophore-conjugated antibodies and other fluorochromes. Much like immunohistochemistry, which produces two- or three ...

The results of bioinformatic and flow cytometry analyses proved that the infiltration ... was added to allow spontaneous differentiation of monocytes into resting (M0) macrophages. FITC-CFSE labeling ...

Methods: Bone marrow-derived macrophages from three wild-type C57BL/6J mice were differentiated (M0) and stimulated with LPS/IFNγ ... with treated macrophages and subsequent analysis by flow cytometry ...

To better understand these processes, flow cytometry is used to phenotype cells by taking their dimensions and assaying their interiors in a streamlined fashion. Flow cytometry is a technique used ...

Granulocyte macrophage colony stimulating factor (GM-CSF ... we used an AAV2-GFP (green fluorescent protein) assay detected with flow cytometry (60–70%). B16-F10 cells were seeded in 48-well plates at ...

Flow cytometry was used to examine the cell surface markers ... analyzed using a FACSC alibur analyzer and Cell Quest software (BD Biosciences). Macrophage polarization (M0, M1, and M2) was identified ...

Flow cytometry allows for the analysis of single cells in a population. This technique is analogous to microscopy, but instead of producing an image, a flow cytometer provides automated ...

Flow cytometry was conducted on a FACSVerse instrument (BD Biosciences ... cells/well in a 96-well plate and either treated with 50 µM SAA1, maintained as M0 macrophages, or polarized towards M1- or ...

F. Flow cytometry analysis of cell surface markers in WT and MSR1 −/− M0 macrophages and M2 (IL‐4) macrophages untreated or stimulated with the MSR1 ligands fucoidan (50 μg/ml, 24 h). Data show ...