The absorbance value was detected at 490 nm under a microplate reader (Biotek, USA). The matrigel invasion assay was performed using a transwell chamber (LABSELECT, China) precoated with matrigel ...
Invasion assays were performed in 24-well plates (Nest) using chambers with 8 µm pore transparent PET filters (LABSELECT, China). Matrigel (Corning ... transferred to the transwell inserts and ...
Different from the migration assay, the invasion assay is conducted in Transwell chambers precoated with Matrigel (at a concentration of 2 mg/mL). The Matrigel is incubated at 37°C for 30 min to form ...
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