PBMC isolation was done following a standard protocol (GE Healthcare, 2010). 600–1,000 µL blood were filled up with 0.14 M NaCl to increase the volume up to 4 mL. 3 mL Ficoll™-Paque Plus (ρ = 1.077 ...
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Subdistal appendages (sDAPs) are centriolar elements that are observed proximal to the distal appendages (DAPs) in vertebrates. Despite the obvious presence of sDAPs, structural and functional ...
RNA granules are protein/RNA condensates. How specific mRNAs are recruited to cytoplasmic RNA granules is not known. Here, we characterize the transcriptome and assembly of P granules, RNA granules in ...