Direct chemical synthesis has been adapted to make oligonucleotides of remarkable length, including an 800 nucleotide green fluorescent protein gene and a 1728 nucleotide bacteriophage DNA polymerase ...

Structural and chemical instability of naked, nonmodified nucleic acids • Availability of conclusive biochemical or in vivo assays for protein activity • Design and selection • Complex folding ...

By employing dialysis with periodic exchange of the feeding solution, we achieved a protein yield of 1.16 mg/mL GFP in the PURE system and extended protein synthesis for at least ... 0.05 mM folinic ...

When grazed by horses, the only difference in blood amino acid levels was a decrease in threonine levels for horses grazing teff. While alfalfa and cool-season grasses were higher in protein and lower ...