4 Researchers load the samples into the wells of a thin polyacrylamide gel, where an electrical current pushes the proteins through the gel matrix; because smaller proteins pass through the gel more ...

Large protein complexes greater than ... 25 °C or on ice for crude extracts) for 1 h. 4| Load the samples directly (with no dye) onto the gel. Carefully layer the mix onto the bottom of the ...

Finally, after the DNA, RNA, or protein molecules have been separated using gel electrophoresis, bands representing molecules of different sizes can be detected.