However, current ubiquitination-derived probes are limited to studying substrate mono- or diubiquitination events. Probes capable of investigating how E3 ligases accommodate E2–Ub conjugates and ...

Generative A.I. chatbots are going down conspiratorial rabbit holes and endorsing wild, mystical belief systems. For some people, conversations with the technology can deeply distort reality.

Upon proteotoxic stress in yeast, the intrinsically disordered protein Roq1 binds the ubiquitin ligase Ubr1 as a pseudosubstrate, thereby modulating the degradation of substrates of the N-degron ...

The SCF (SKP1/CUL1/F-box protein) ubiquitin ligase complex plays a protective role against external stress, such as ultraviolet irradiation. The emergence of substrates activates SCF through ...

Initially, the ubiquitin substrate is activated by the E1-activating enzyme. Then activated-ubiquitin is transferred to the E2 conjugating enzyme, and finally, the E3-ligase transfers ubiquitin to the ...

Human proteins undergo a variety of chemical modifications following their synthesis. These modifications regulate their structure, function, and stability. Researchers from the Bhogaraju Group at ...

Article Title A ubiquitin-specific, proximity-based labeling approach for the identification of ubiquitin ligase substrates ...

During ubiquitination, a group of enzymes called E3 ubiquitin ligases attach a small protein called ubiquitin to other proteins. This tagging, in turn, helps determine the fate of the targeted ...

Identifying the substrates of ubiquitin protein ligase (E3) and deubiquitinases (DUB) contributes to the discovery of potential therapeutic targets for diseases. However, experimental identification ...