Huh7 is an immortalised cell line that is grown in the laboratory for research purposes. It is described as a hepatocyte-derived carcinoma cell line, originally taken from a liver tumor in a 57-year-old Japanese male in 1982. [1] It is used extensively in hepatitis C and dengue virus research. [2] [3] Huh7 cells have been instrumental in ...

Apr 4, 2012 · Stable HepG2- and Huh7-based human hepatoma cell lines for efficient regulated expression of infectious hepatitis B virus. J. Hepatol. 45:636-645(2006) PubMed= 20215515 ; DOI= 10.1158/0008-5472.CAN-09-3458 ; PMCID= PMC2881662

Huh-7 is an immortal cell line composed of epithelial-like, tumorigenic cells. The majority of Huh-7 cells show a chromosome number between 55 and 63 (mode 60) and are highly heterogeneous. These cells are adherent to the surface of flasks or plates and typically grow as 2D monolayers.

HuH-7 Cells should be cultured in DMEM supplemented with 10% FBS. Cells should be grown at 37 °C in a 5% CO2 setting. To subculture: Renew medium 2-3 times a week, or as needed according to media pH, and maintain cell confluency between 30-90%.

HuH-7 is a well-known cell line used as a model for investigating both hepatoma and HCV. Tumor cells acquire genetic alterations during their evolution, which underlie cancer development, progression and drug resistance [8].

Huh7 cells are notable for their ability to produce infectious hepatitis C virus particles in vitro, which makes them a valuable tool for studying the biology of hepatitis C virus infection and for evaluating the effectiveness of new anti-viral drugs.

To date, the human hepatoma cell line Huh7 and its derivatives is the only cell line that robustly replicates HCV. However, even different subclones and passages of this single cell line exhibit tremendous differences in HCV replication efficiency.

Apr 13, 2021 · Huh-7 is an immortal human hepatic cell line that is highly heterogenous. They are tumorigenic, adherent cells that normally generate two-dimensional cell monolayers.

May 17, 2018 · HuH-7 is a well-known cell line used as a model for investigating both hepatoma and HCV. Tumor cells acquire genetic alterations during their evolution, which underlie cancer development, progression and drug resistance [8].

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