The Thermo Scientific™ ™Pierce BCA Protein Assay Kit is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein. This method combines the well-known reduction of Cu+2 to Cu+1 by protein in

The bicinchoninic acid assay (BCA assay), also known as the Smith assay, after its inventor, Paul K. Smith at the Pierce Chemical Company, [1] now part of Thermo Fisher Scientific, is a biochemical assay for determining the total concentration of protein in a solution (0.5 μg/mL to 1.5 mg/mL), similar to Lowry protein assay, Bradford protein ...

The bicinchoninic acid (BCA) assay is one of many ways to measure the total protein concentration of an unknown sample. In this assay, copper (II) [Cu 2+ ] is reduced to copper (I) [Cu 1+ ] in the presence of peptides in alkaline solutions.

The bicinchoninic acid (BCA) protein assay is generally preferred over other concentration assays because it is compatible with most protein sample types and provides a more accurate...

Here, we describe an NADH-independent enzymatic assay allowing rapid, selective, and sensitive quantification of L-lactate down to the pmol range.

BCA Protein Assay. The BCA method is one of the most widely used assays for determining protein concentration. It is a highly sensitive method with a wide linearity range and less protein-to-protein variation than other methods. Using the 8-Cell Changer Accessory combined with the built-in Protein BCA method included in the BioMate 160

Download scientific diagram | Bicinchoninic acid (BCA) assay standard curve for serum samples analysis. The blue dotted line showed trend line robust fit.

The BCA protein assay was first described in 1985 to determine protein concentration with the use of bicinchoninic acid (BCA). In common with the Lowry assay, the biuret reaction is the first step in the reaction that takes place in the BCA assay. In this reaction, protein reduces Cu2+ to Cu+ in an alkaline environment. In the second step,

The BCA Protein Assay from G-Biosciences comprises two assay formats based on the dynamic range required to measure the concentration of the protein of interest. It can be performed either in a 1mL cuvette assay or in micro-wells.

Bicinchoninic acid (BCA) assay or Smith assay is a copper-based colorimetric assay for total protein quantification. BCA rely on the formation of a Cu 2 + –protein complex in a basic environment, followed by reduction of the Cu 2 + to Cu + (Smith et al., 1985).