Apr 22, 2024 · The Xpert MTB/RIF assay is a nucleic acid amplification (NAA) test that uses a disposable cartridge with the GeneXpert Instrument System. A sputum sample is collected from the patient with suspected TB.

The Xpert MTB/RIF test provides fast and accurate detection of Mycobacterium Tuberculosis (MTB) and Rifampicin (RIF) resistance simultaneously from patient samples

The Xpert MTB/RIF is a cartridge-based nucleic acid amplification test (NAAT) for rapid tuberculosis diagnosis and rapid antibiotic sensitivity test. It is an automated diagnostic test that can identify Mycobacterium tuberculosis (MTB) DNA and resistance to rifampicin (RIF).

The Xpert® MTB/RIF test, performed on the GeneXpert® Instrument Systems, is a qualitative, nested real-time polymerase chain reaction (PCR) in vitro diagnostic test for the detection of Mycobacterium tuberculosis complex DNA in raw sputum or concentrated sputum sediment prepared from induced or expectorated sputum.

In 2017, China published the standard WS288-2017 Tuberculosis Diagnosis, which includes a positive MTB nucleic acid test as one of the diagnostic criteria for tuberculosis. The advent of the GeneXpert MTB/RIF (GeneXpert) cassette test represents a …

Xpert® MTB/RIF revolutionized the management of Mycobacterium tuberculosis (MTB) infections by providing faster and more accurate MTB diagnosis that detects MTB and rifampicin (RIF) resistance simultaneously. 2017 WHO endorsed MTB Ultra as a replacement to MTB/RIF and smear microscopy.

Jul 25, 2019 · The GeneXpert MTB/RIF system is a fully automated nested real-time PCR system, which detects MTBC DNA in smear positive and negative sputum samples. It simultaneously identifies mutations in the rpoB gene, which is associated with rifampicin resistance.

The GeneXpert MTB/RIF assay is a novel integrated diagnostic device that performs sample processing and heminested real-time PCR analysis in a single hands-free step for the diagnosis of tuberculosis and rapid detection of RIF resistance in clinical specimens (3, 9).

The Xpert MTB/RIF assay aids in the prompt diagnosis of TB and RMP-resistant disease. RMP resistance most often coexists with isoniazid (INH) resistance; TB that is resistant to both drugs is multidrug-resistant (MDR)† TB.

GeneXpert MTB/RIF assay is a nucleic acid amplification (NAA) test which simultaneously detects DNA of Mycobacterium tuberculosis complex (MTBC) and resistance to rifampin (RIF) (i.e. mutation of the rpoB gene) in less than 2 hours.