A study of PCR performance and y-intercept values on nine different platforms using the instrument’s default settings vs manually tuning baseline settings and thresholds. In most …

Real-time PCR, also called quantitative PCR or qPCR, can provide a simple and elegant method for determining the amount of a target sequence or gene that is present in a sample. Its very …

Oct 17, 2022 · Real-time PCR data are often represented in a graph called an amplification plot (Figure 1). The vertical axis is in fluorescent units, and the measurement is often called ΔRn, …

Jun 18, 2015 · A PCR amplification curve which looks like Figure 1 is generally a sign of a “healthy,” good PCR reaction. As a direct measure of that, we could actually go in and …

Dec 12, 2024 · Real-time PCR data analysis is a critical step in understanding the efficiency and specificity of the PCR reaction. By understanding the key concepts, tools, and techniques …

So, I will break down the three areas of qPCR interpretation that we’re going to detail in this article: Understanding the qPCR chart. The importance of PCR efficiency and how to calculate …

Fluorescent Quantitative PCR (qPCR) technology allows precise tracking of fluorescence signal changes during the PCR process in real-time. In the context of fluorescent quantitative PCR …

May 5, 2011 · If you run quantitative real-time PCR (qPCR) assays, the chances are that you’ve seen some strange looking amplification plots. When something looks off, it’s a red flag to start …

Real-time PCR has become a widely used technique for a variety of applications, such as gene expression, mutational analysis, and pathogen detection. Many biotechnology companies offer …

Throughout this guide, the factors that contribute to variations in the measurement of nucleic acid using PCR or qPCR have been highlighted. Each of these factors should be optimized to …