Carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) is an amine-reactive, cell-permeable dye generally used in animal cell proliferation research. CFDA-SE is a modified CFSE with two hydroxyl groups on its fluorescein moiety replaced with acetates.

Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye. CFSE is cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules, [1] notably, to intracellular lysine residues and other amine sources.

The Vybrant™ CFDA SE Cell Tracer Kit contains convenient single-use vials of dry dye to permit small-scale experiments without preparing excess quantities of dye, thereby extending the shelf life of the dye not dissolved in solution.

CFDA, SE is a fixable-cell-permeant, fluorescein-based tracer for very long-term cell labeling. The dye is also available in our convenient, easy-to-use Vybrant CFDA-SE Cell Tracer Kit (V12883).

CFDA-SE (5(6)-carboxyfluorescein diacetate succinimidyl ester) is a stable, cell-permeable diacetate precursor to CFSE. Upon diffusion into the cell, intracellular esterases cleave the acetate group to generate CFSE, which interacts with cellular amines via its succinimidyl groups to generate a highly fluorescent green dye that is impermeant to ...

CFSE (or CFDA-SE, carboxyfluorescein diacetate, succinimidyl ester) is a fluorescein derivative which, when added to cells at particular time, causes them to fluoresce. From: Comprehensive Biotechnology (Second Edition), 2011

Nov 18, 2000 · CFDA-SE labeling is somewhat toxic and can induce growth arrest and apoptosis in some cell types – therefore, it is important to find the lowest acceptable labeling concentration and check the viability after labeling.

Carboxyfluorescein diacetate succinimidyl ester (5(6)-CFDA, SE; C1157) is commonly referred to as CFSE. Among its many applications, the two most prominent are ex vivo labeling of cells for adoptive transfer and flow cytometric tracking of cell division by label partition analysis.

Carboxyfluorescein succinimidyl ester (CFSE) is an effective and popular means to monitor lymphocyte division 1-3. CFSE covalently labels long-lived intracellular molecules with the fluorescent dye, carboxyfluorescein.

Oct 25, 2024 · CFDA-SE is a fluorescent dye that can penetrate the cell membrane. It can react with the free amine group in the cytoskeleton protein inside the cell, and finally form a protein complex with fluorescence.