
HepG2 knockout (KO) Cell Line - Ubigene
Aug 13, 2020 · HepG2 cells paired with CRISPR/Cas9 offer a robust model for researching hepatitis, genetic diseases, and liver cancer.
The Curious Case of the HepG2 Cell Line: 40 Years of Expertise
Dec 4, 2021 · In this regard, we set out to collect up-to-date information on the HepG2 cell line to assess whether the level of heterogeneity of the cell line allows in vitro biomedical studies as a model with guaranteed production and quality.
Hep G2 Cell Line: Liver Cancer Research Applications with Gene
Oct 10, 2024 · Gene Knockout (KO): Precise deletion of target genes in Hep G2 aid functional research related to liver diseases and cancer. Point Mutation & Knock-In (KI): Smart-CRISPR™ enables high-efficiency homologous recombination in Hep G2 cells to introduce precise mutations or gene insertions.
NSMAF Knockout cell line (Hep G2) | Ubigene
The NSMAF Knockout cell line (Hep G2) is constructed based on (1) the optimal design of the Red Cotton System, (2) CRISPR-U™ technology with efficiency 10-20 times higher than traditional methods, and (3) our exclusive EZ-editor™ product series. This cell line is validated by Sanger sequencing.
Functional analysis and drug response to zinc and D-penicillamine …
HepG2 cells are widely used for analysis of Cu toxicity and apoptosis[36,37]. By using ATP7B KO cells derived from HepG2, we assessed viability of hepatic cells without interference by intrinsic ATP7B or impairment by hybrid protein sequences[31,37] reflecting …
Inhibition of Glypican-3 Cleavage Results in Reduced Cell …
Feb 1, 2023 · GPC3 KO cells were generated using HepG2 cells and Crispr/Cas9 gene editing with guide RNA and a PAM site near the GPC3 furin cleavage site, which resulted in a premature stop codon in exon 4 of GPC3.
Cereblon deficiency ameliorates carbon tetrachloride-induced …
Jul 30, 2024 · We established a CRBN-knockout (KO) HepG2 cell line and examined its effect on CCl 4-induced hepatocellular damage. CRBN-KO cells exhibited reduced sensitivity to CCl 4 -induced cytotoxicity, as evidenced by decreased levels of apoptosis markers, such as cleaved caspase-3, and aspartate aminotransferase (AST) and alanine aminotransferase (ALT ...
CRISPR/Cas9-mediated knock-out of AGXT1 in HepG2 cells as a …
Nov 1, 2022 · CRISPR/Cas9 technology leads to effective editing of AGXT1 gene in HepG2 cells. AGXT1 -KO cells recapitulate the main biochemical features of AGXT1 deficiency. Response to pyridoxine of common PH1-variants mirrors clinical evidence. AGXT1 -KO cells are a new model for pathogenesis studies and drug discovery.
Knockouts of CYP51A1, DHCR24, or SC5D from cholesterol …
We developed HepG2 cell models with knockouts (KOs) for three enzymes of cholesterol synthesis, each accumulating specific sterols. Surprisingly, KOs of CYP51, DHCR24, and SC5D shared only 9% of differentially expressed genes.
Leukocyte cell-derived chemotaxin 2 is an antiviral regulator acting ...
Jun 8, 2022 · HepG2-SHP2-KO cells showed significantly enhanced Poly(I:C)-induced IFNB1 expression compared with HepG2-WT cells (Fig. 6d). However, we observed that r-LECT2 no longer activated innate immune responses in the absence of SHP2 (Fig. 6d, e ).