In this study we evaluated the concordance of HER2 status between bright-field dual in situ hybridization (DISH), fluorescence in situ hybridization (FISH) and HER2 immunohistochemistry (IHC) performed in formalin-fixed CBs of recurrent and metastatic breast cancers.

Feb 26, 2020 · Cases with false-negative HER2 dual in situ hybridization (DISH) results. A, Case 33. HER2 DISH has faintly perceptible black HER2 signals, some of which are located outside of nuclei. Many nuclei lack black signals, reflecting a technical problem. B, Case 33: corresponding HER2 fluorescence in situ hybridization (FISH) with increased red HER2 ...

The VENTANA HER2 Dual ISH DNA Probe Cocktail contains HER2 probes (labeled with the hapten dinitrophenyl or DNP) and Chromosome 17 probes (labeled with the hapten digoxigenin or DIG) formulated in a formam ide-based buffer.

Jan 1, 2014 · To compare the INFORM HER2 bright-field dual in situ hybridization (DISH) DNA probe cocktail assay with the PathVysion fluorescence in situ hybridization (FISH) assay on 103 invasive breast carcinomas with a 2+ score on immunohistochemistry (IHC).

The HER2 DISH assay, utilizing 10 % buffered formalin-fixed CB, would be a reliable and ideal method to assess the HER2 gene status of breast cancer cytological specimens. Keywords: Breast cancer, Cytology, Cell block, HER2 gene, DISH

The new VENTANA HER2 Dual ISH Probe Cocktail is a fully automated, ready-to-use brightfield solution for determining HER2 gene amplification. VENTANA HER2 Dual ISH helps identify breast cancer patients eligible for treatment with HERCEPTIN ® (trastuzumab).

Dec 1, 2020 · Accurate HER2 testing in breast cancer is crucial for appropriate precision therapy. HER2 testing is most commonly accomplished by a combination of immunohistochemistry and in situ hybridization techniques, as gene amplification is closely tied to protein overexpression.

Jul 25, 2023 · Dual-color dual in situ hybridization (D-DISH), a recently introduced fully automated assay for HER2/neu evaluation on light microscopy, has several advantages over fluorescence in situ hybridization (FISH). Objective.—

Dual-color dual-hapten in situ hybridization (D-DISH) is a new test for assessment of HER2/neu gene overexpression on light microscopy. Aims: This was a validation study for D-DISH in our laboratory and was conducted to study the concordance between fluorescence in situ hybridization (FISH) and D-DISH for HER2/neu testing in breast cancer.

Sep 22, 2019 · In the current study, the authors evaluated the concordance of HER2 status between bright-field dual in situ hybridization (DISH), fluorescence in situ hybridization (FISH), and HER2 immunohistochemistry (IHC) performed on formalin-fixed CBs of recurrent and metastatic breast cancers.