In vitro transcription (IVT) is a simple procedure that allows for template-directed synthesis of RNA molecules of any sequence from short oligonucleotides to those of several kilobases in μg to mg quantities [1].

Here, we describe the advances that have supported the adoption of IVT mRNA in the clinics, including optimization of the IVT mRNA structural components, synthesis, and lastly concluding with different classes of IVT RNA.

May 21, 2022 · Accordingly, IVT mRNA is performed to complete the transcription of RNA in vitro by stimulating the mechanism of eukaryotic mRNA synthesis to ensure the expression of mRNA in vivo (Fig. 3)....

Nov 10, 2022 · In vitro transcription (IVT) is a DNA-templated process for synthesizing long RNA transcripts, including messenger RNA (mRNA).

With the development of in vitro transcription (IVT) methods, synthetic mRNA has become an emerging class of gene therapy. IVT mRNA has several advantages over conventional pDNA for the expression of target proteins. mRNA does not require nuclear localization to …

3 days ago · In vitro transcription (IVT) synthesizes mRNA using a DNA-dependent RNA polymerase like T7, SP6, or T3. The reaction mixture includes ribonucleotide triphosphates (NTPs), a buffer system, and magnesium ions essential for polymerase activity. To improve IVT efficiency, modified nucleotides enhance stability and reduce immune recognition.

In the shadow of DNA-based gene therapy, a few scientists experimented with in vitro-transcribed (IVT) mRNA to express a therapeutic protein. Early on, the IVT mRNA was injected into frog oocytes or transfected into cultured cells.

Sep 19, 2014 · In vitro transcribed (IVT) mRNA has recently come into focus as a potential new drug class to deliver genetic information. Such synthetic mRNA can be engineered to transiently express proteins...

In vitro transcribed (IVT) mRNA has been widely used for various purposes, such as vaccines, protein replacement, induced pluripotent stem cells, and cancer immunotherapy.

Mar 25, 2024 · In vitro transcribed mRNA has proven its value for both basic research and the rapid development of lifesaving therapeutics, thanks to its relatively simple manufacturing process. IVT mRNA synthesis is a flexible, cell-free, and rapidly scalable method.