6 days ago · To evaluate mRNA LNP efficacy and biodistribution in vivo, we use C57BL/6 mice (female 6–8 weeks old, weight 18–21 g), intravenously injected with various FLuc mRNA LNP formulations at a dose ...

Aug 10, 2021 · In this Review, we discuss the design of lipid nanoparticles for mRNA delivery and examine physiological barriers and possible administration routes for lipid nanoparticle–mRNA systems.

Dec 7, 2021 · After decades of extensive fundamental studies and clinical trials, lipid nanoparticles (LNPs) have demonstrated effective mRNA delivery such as the Moderna and Pfizer-BioNTech vaccines fighting against COVID-19.

Lipid nanoparticles (LNPs), based on ionizable lipids, are the most commonly used messenger RNA (mRNA) delivery vectors and play a crucial role in the research and clinical translation of mRNA therapy.

Dec 1, 2024 · Lipid nanoparticles (LNPs) used for nonviral gene delivery have achieved significant success, particularly in COVID-19 mRNA vaccines. LNPs are routinely characterized by their particle size, polydispersity, and mRNA loading efficiency.

The findings underscore the potential of hydroxycholesterol modifications in LNP formulations to optimize mRNA delivery systems, which could have profound implications for developing advanced immunotherapies and vaccines.

3 days ago · The COVID-19 pandemic highlighted the effectiveness and speed of mRNA-Lipid Nanoparticles (LNP) vaccine platforms developed by Pfizer/BioNTech and Moderna in addressing emerging crises. 1 Currently, three mRNA-LNP drugs have been approved by the Food and Drug Administration (FDA), all of which are prophylactic vaccines (Table 1).The advantages of mRNA-LNP therapeutics include safety ...

Mar 4, 2025 · Lipid nanoparticle (LNP)-mRNA vaccines have demonstrated protective capability in combating SARS-CoV-2. Their extensive deployment across the global population leads to the broad presence of T ...

Aug 1, 2024 · To overcome the biological barriers of mRNA, lipid nanoparticles (LNPs) have emerged as promising mRNA-packing nanotechnology, gaining revolutionary advances attributed to the widespread applications of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mRNA-LNP vaccines [6], [7].

2 days ago · LNPs are non-viral delivery vehicles obtained by the self-assembly of lipids during the rapid mixing of an aqueous phase containing mRNA with an organic phase containing lipids. During this process, mRNA is encapsulated within the LNP due to …